超高灵敏度荧光显微镜及其在生物学中的应用

http://www.microimage.com.cn(2010-06-02 10:41:49)

李勤,俞信,胡新奇,马瑜,张同存,曹恩华超高灵敏度荧光显微镜及其在生物学中的应用[J]. 光学学报, 2000, 20(4): 533537

 

超高灵敏度荧光显微镜及其在生物学中的应用  

Ultra-High Sensitivity Fluorescence Microscope and Its Application in Biology

 

李勤 俞信 胡新奇 马瑜 张同存 曹恩华

 

 

    介绍了在原有数字化高灵敏度荧光显微镜的基础上研制的超高灵敏度荧光显微镜, 对荧光实现106数量级的增益, 可获得细胞的光子灵敏显微图像, 并结合图像融合技术, 实现极微弱荧光图像的定位显示。 采用具有视频速率的图像采集系统, 可用于研究某区域荧光强度随时间变化的动力学过程。 将该显微镜应用在生物医学领域的细胞凋亡的研究, 观察到As2O3诱导MGC-803细胞凋亡中Ca2+浓度升高, 预示着Ca2+可能是由As2O3启动, 促发MGC-803细胞凋亡的又一重要机制。

 

    超高灵敏度荧光显微镜 光子灵敏显微图像 图像融合 细胞凋亡 ultra-high-sensitivity fluorescence microscope photon sensitive image image fusion apoptosis.
 

AbstractBased on the digital high-sensitivity fluorescence microscope, the ultra-high-sensitivity fluorescence microscope is developed. The system can enhance the fluorescence intensity more than 106 and get the photon sensitive image of ultra-weak fluorescence. The accurate position of ultra-weak fluorescence can be confirmed by image fusion methods. Because the speed of image acquired system is 25 frame/s, the dynamic characters of fluorescence intensity can be investigated. Using the novel microscope to study the apoptosis of MGC-803 cells induced by As2O3, the density of Ca2+ in apoptotic cells obviously increased. Ca2+ is probably one of important roles in the process of MGC-803 apoptosis induced by As2O3.

 

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